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NMR of 133Cs+ in stretched hydrogels: One-dimensional, z- and NOESY spectra, and probing the ionís environment in erythrocytes

Philip W. Kuchel
Organization: The University of Sydney
Department: Department of Biochemistry
URL: http://www.bioeng.auckland.ac.nz/MCF/invited_users/philip_kuchel.htm
Dmitry Shishmarev
Max Puckeridge
Malcolm H. Levitt
Christoph Naumann
B. E. Chapman
Journal / Anthology

Journal of Magnetic Resonance
Year: 2015
Volume: 261
Page range: 110-120

133Cs nuclear magnetic resonance (NMR) spectroscopy was conducted on 133Cs+ in gelatin hydrogels that were either relaxed or stretched. Stretching generated a septet from this spin-7/2 nucleus, and its nuclear magnetic relaxation was studied via z-spectra, and two-dimensional nuclear Overhauser (NOESY) spectroscopy. Various spectral features were well simulated by using Mathematica and the software package SpinDynamica. Spectra of CsCl in suspensions of human erythrocytes embedded in gelatin gel showed separation of the resonances from the cation inside and outside the cells. Upon stretching the sample, the extracellular 133Cs+ signal split into a septet, while the intracellular peak was unchanged, revealing different alignment/ordering properties of the environment inside and around the cells. Differential interference contrast light microscopy confirmed that the cells were stretched when the overall sample was elongated. Analysis of the various spectral features of 133Cs+ reported here opens up applications of this K+ congener for studies of cation-handling by metabolically-active cells and tissues in aligned states.