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Human Oocyte Vitrification: The permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification
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Mathematica was used to perform cell dynamic modeling. The program was written by Steven F. Mullen, Ph.D.
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Objectives To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes.
Design An incomplete randomized block design was used for this study.
Setting A University-affiliated assisted reproductive center.
Patients Women undergoing assisted reproduction in the Center for Reproductive Medicine at Shandong University.
Interventions Oocytes were exposed to 1.0 molar ethylene glycol in a single step, and photographed during subsequent volume excursions.
Main outcome measures A 2-parameter model was employed to estimate the permeability to water and EG.
Results Water permeability ranged from 0.15 to 1.17 µm/(min·atm), and ethylene glycol permeability ranged from 1.5 to 30 µm/min between 7 °C at 36 °C. The activation energies for water and ethylene glycol permeability were 14.42 Kcal/mol and 21.20 Kcal/mol, respectively.
Conclusions Despite the lower permeability of human MII oocytes to ethylene glycol compared to previously published values for propylene glycol and dimethylsulfoxide, methods to add and remove human oocytes with a vitrifiable concentration of ethylene glycol can be designed which prevent excessive osmotic stress and minimize exposure to high concentrations of this compound.
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Vitrification, ethylene glycol, human, oocytes, permeability, computer modeling
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