|
|
|
|
|
|
 |
|
|
K-39 nuclear magnetic resonance and a mathematical model of K+ transport in human erythrocytes
|
|
|
|
|
|
| Organization: | The University of Sydney |
| Department: | Department of Biochemistry |
|
|
|
|
|
|
| EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS |
|
|
|
|
|
|
K-39 nuclear magnetic resonance was used to measure the efflux of K+ from suspensions of human erythrocytes [red blood cells (RBCs)], that occurred in response to the calcium ionophore, A23187 and calcium ions; the latter activate the Gairdos channel. Signals from the intra- and extracellular populations of (39)K(+)were selected on the basis of their longitudinal relaxation times, T-1, by using an inversion- recovery pulse sequence with the mixing time, tau(1), chosen to null one or other of the signals. Changes in RBC volume consequent upon efflux of the ions also changed the T-1 values so a new theory was implemented to obviate a potential artefact in the data analysis. The velocity of the K(+)efflux mediated by the Gairdos channel was 1.19 +/- 0.40 mmol (L RBC)(-1) min(-1) at 37 degrees C.
|
|
|
|
|
|
|
|
|
|
|
|
erythrocytes, Gárdos channel, membrane transport, nuclear magnetic resonance, red blood cells
|
|
|
|
|
|
|
| | | | | |
|
For the newest resources, visit Wolfram Repositories and Archives »
