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Human Oocyte Vitrification: The permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification

Steven F. Mullen
Mei Li
Yuan Li
Zi-Jiang Chen
John K. Critser
Journal / Anthology

Fertility and Sterility
Year: 2008
Volume: 89
Issue: 6
Page range: 1812-1825

Mathematica was used to perform cell dynamic modeling. The program was written by Steven F. Mullen, Ph.D.

Objectives To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes.

Design An incomplete randomized block design was used for this study.

Setting A University-affiliated assisted reproductive center.

Patients Women undergoing assisted reproduction in the Center for Reproductive Medicine at Shandong University.

Interventions Oocytes were exposed to 1.0 molar ethylene glycol in a single step, and photographed during subsequent volume excursions.

Main outcome measures A 2-parameter model was employed to estimate the permeability to water and EG.

Results Water permeability ranged from 0.15 to 1.17 m/(minatm), and ethylene glycol permeability ranged from 1.5 to 30 m/min between 7 C at 36 C. The activation energies for water and ethylene glycol permeability were 14.42 Kcal/mol and 21.20 Kcal/mol, respectively.

Conclusions Despite the lower permeability of human MII oocytes to ethylene glycol compared to previously published values for propylene glycol and dimethylsulfoxide, methods to add and remove human oocytes with a vitrifiable concentration of ethylene glycol can be designed which prevent excessive osmotic stress and minimize exposure to high concentrations of this compound.

*Science > Biology
*Science > Medicine

Vitrification, ethylene glycol, human, oocytes, permeability, computer modeling