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Title

K-39 nuclear magnetic resonance and a mathematical model of K+ transport in human erythrocytes
Authors

AD Maher
B. E. Chapman
Philip W. Kuchel
Organization: The University of Sydney
Department: Department of Biochemistry
URL: http://www.bioeng.auckland.ac.nz/MCF/invited_users/philip_kuchel.htm
Journal / Anthology

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Year: 2006
Volume: 35
Issue: 4
Page range: 293-301
Description

K-39 nuclear magnetic resonance was used to measure the efflux of K+ from suspensions of human erythrocytes [red blood cells (RBCs)], that occurred in response to the calcium ionophore, A23187 and calcium ions; the latter activate the Gairdos channel. Signals from the intra- and extracellular populations of (39)K(+)were selected on the basis of their longitudinal relaxation times, T-1, by using an inversion- recovery pulse sequence with the mixing time, tau(1), chosen to null one or other of the signals. Changes in RBC volume consequent upon efflux of the ions also changed the T-1 values so a new theory was implemented to obviate a potential artefact in the data analysis. The velocity of the K(+)efflux mediated by the Gairdos channel was 1.19 +/- 0.40 mmol (L RBC)(-1) min(-1) at 37 degrees C.
Subject

*Science > Biochemistry
Keywords

erythrocytes, Gárdos channel, membrane transport, nuclear magnetic resonance, red blood cells