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Title

Interaction of Lipophilic Ions with the Plasma Membrane of Mammalian Cells Studied by Electrototation
Authors

M. Kürschner
K. Nielsen
C. Andersen
V. Sukhorukov
W. Schenk
B. Benz
U. Zimmermann
Journal / Anthology

Biophysical Journal
Year: 1998
Volume: 74
Page range: 3031-3043
Description

The electrical properties of biological and artificial membranes were studied in the presence of a number of negatively charged tungsten carbonyl complexes, such as [W(CO)5(CN)]-, [W(CO)5(NCS)]-, [W2(C))10(CN)]-, and [W(CO)5(SCH2C6H5)]-, using the single-cell electrorotation and the charge-pulse relaxation techniques. Most of the negatively charged tungsten complexes were able to introduce mobile charges in to the membranes, as judged from electrorotation spectra and relaxation experiments. This means that the tungsten derivatives act as lipophilic anions. They greatly contributed to the polarizability of the membranes and led to a marked dielectric dispersion (frequency dependence of the membrane capacitance and conductance). The increment and characteristic frequency of the dispersion reflect the structure, environment, and mobility of the charged probe molecule in electrorotation experiments with biological membranes. The partition coefficients and the translocation rate constants derived from the electrorotation experiments with biological membranes. The partition coefficients and the translocation rate constants derived from the electorotaion spectra of cells agreed well with the corresponding data obtained from charge-plus experiments on artificial lipid bilayers.
Subject

*Science > Biology